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45-462 (1ug/ml) staining of Rat Testes lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-462 staining of paraffin embedded Mouse Testis. <strong>This data is from a previous batch, not on sale.</strong>
45-462 (1ug/ml) staining of Rat Testes lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

DAZL Antibody

CATALOG NUMBER: 45-462

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Rat
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-GNGPQKKSVDR
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 38kDa band observed in Rat Testis lysates (calculated MW of 31.4kDa according to NP_001102884.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-1ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse Ovary for this particular batch.
Positive Control 1:
Cat. No. 1316 - Human Ovary Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DAZL
Additional Names:
DAZL, deleted in azoospermia-like , HGNC:2685, DAZH, DAZL1, DAZLA, MGC26406, SPGYLA, deleted in azoospermia-like autosomal, germline specific RNA binding protein, spermatogenesis gene on the Y-like autosomal
Protein Accession Number:
NP_001342.2
Protein Gi Number:
25121978
Ncbi Gene Id Number:
1618
Background Reference 1:
Cauffman G, Van de Velde H, Liebaers I, Van Steirteghem A. DAZL expression in human oocytes, preimplantation embryos and embryonic stem cells. Mol Hum Reprod. 2005 Jun;11(6):405-411. Epub 2005 May 6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-462

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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