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Western blot analysis of extracts of mouse brain, using DBH antibody (18-778) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human stomach using DBH antibody (18-778) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using DBH antibody (18-778) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of mouse brain, using DBH antibody (18-778) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.

DBH Antibody

CATALOG NUMBER: 18-778

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human DBH (NP_000778.3).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:1000
IHC: 1:50 - 1:100
Positive Control 1:
Mouse brain
Predicted Molecular Weight:
Observed: 79kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
DBH
Additional Names:
DBH, dopamine beta-hydroxylase (dopamine beta-monooxygenase), dopamine beta-hydroxylase, DBM
Ncbi Gene Id Number:
1621
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-778

List Size:
100 uL

List Price:

$436.00

Datasheet
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