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Western blot analysis of extracts of various cell lines, using DBI antibody (14-894) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human prostate using DBI Antibody (14-894) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using DBI Antibody (14-894) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using DBI antibody (14-894). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using DBI antibody (14-894) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

DBI Antibody

CATALOG NUMBER: 14-894

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-114 of human DBI (NP_001171513.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
HT-29
Positive Control 2:
THP-1
Positive Control 3:
MCF7
Positive Control 4:
NCI-H460
Positive Control 5:
A673
Positive Control 6:
U-937
Predicted Molecular Weight:
Observed: 11kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
DBI
Additional Names:
DBI, ACBP, Acyl-CoA-binding protein, CCK-RP, Diazepam-binding inhibitor, ACBD1, GABA receptor modulator, EP, Endozepine
Ncbi Gene Id Number:
1622
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-894

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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