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Western blot analysis of DBX1 in mouse kidney tissue lysate with DBX1 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of DBX1 in human kidney tissue with DBX1 antibody at 2.5 μg/mL.
Immunofluorescence of DBX1 in human kidney tissue with DBX1 antibody at 20 μg/mL.
Western blot analysis of DBX1 in mouse kidney tissue lysate with DBX1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

DBX1 Antibody

CATALOG NUMBER: 6455

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (87%)
Immunogen:
DBX1 antibody was raised against a 15 amino acid synthetic peptide near the center of human DBX1.
The immunogen is located within amino acids 100 - 150 of DBX1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
DBX1 antibody can be used for detection of DBX1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
BX1 antibody is predicted to not cross-react with other DBX protein family members
Positive Control 1:
Cat. No. 1405 - Mouse Kidney Tissue Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Purification:
DBX1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
DBX1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
DBX1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DBX1
Additional Names:
DBX1 Antibody: Homeobox protein DBX1, Developing brain homeobox protein 1
Protein Accession Number:
NP_001025036
Protein Gi Number:
304434796
Ncbi Gene Id Number:
120237
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DBX1 Antibody: DBX1 is implicated in patterning the central nervous system during embryogenesis. DBX1 homeodomain transcription factor is expressed in progenitors which are critical in establishing cell fate allocation and cell diversity. DBX1 has a key role in regulating the distinct phenotypic features that distinguish two major classes of ventral interneurons, V0 and V1 neurons. DBX1-derived neurons are essential for the transgenic expression and generation of respiratory behavior both in vitro and in vivo.
Background Reference 1:
Griveau A, Borello U, Causeret F, et al. A novel role for Dbx1-derived Cajal-Retzius cells in early regionalization of the cerebral cortical neuroepithelium. PLoS Biol. 2010; 8(7):e1000440
Background Reference 2:
Causeret F, Ensini M, Teissier A, et al. Dbx1-expressing cells are necessary for the survival of the Mammalian anterior neural and craniofacial structures. PLoS One 2011; 6:e19367.
Background Reference 3:
Pierani A, Moran-Rivard L, Sunshine MJ, et al. Control of interneuron fate in the developing spinal cord by the progenitor homeodomain protein Dbx1. Neuron 2001; 29:367-84
Background Reference 4:
Gray PA, Hayes JA, Ling GY, et al. Developmental origin of preBotzinger complex respiratory neurons. J. Neurosci. 2010; 30:14883-95.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6455

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$65 Standard Overnight flat rate for as many products shipped within the United States.

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