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Western blot analysis of WDR18 in rat lung tissue lysate with WDR18 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of DBX2 in human kidney tissue with DBX2 antibody at 5 μg/mL.
Immunofluorescence of DBX2 in human kidney tissue with DBX2 antibody at 20 μg/mL.
Western blot analysis of WDR18 in rat lung tissue lysate with WDR18 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

DBX2 Antibody

CATALOG NUMBER: 6873

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
DBX2 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human DBX2.
The immunogen is located within amino acids 30 - 80 of DBX2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
DBX2 antibody can be used for detection of DBX2 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
DBX2 antibody is human specific. At least two isoforms of DBX2 are known to exist; this antibody will only detect the shortest isoform.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Purification:
DBX2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
DBX2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
DBX2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DBX2
Additional Names:
DBX2 Antibody: Homeobox protein DBX2, Developing brain homeobox protein 2
Protein Accession Number:
NP_001004329
Protein Gi Number:
116174748
Ncbi Gene Id Number:
440097
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DBX2 Antibody: DBX2 is a member of the developing brain homeobox (DBX) protein family, but while the related protein DBX1 is expressed in various regions of the developing brain, DBX2 shows a more restricted pattern of expression in the brain, and is also expressed in some mesenchymal cells such as limb buds and tooth germs. It is thought that DBX1 and DBX2 promote the development of a subset of interneurons, some of which help mediate left-right coordination of locomotor activity. In Xenopus, DBX2 is involved in primary neurogenesis and early neural plate patterning, and is thought to act as a cross-repressive partner of NKX6-2 in the patterning of the ventral neural tube.
Background Reference 1:
Shoji H, Ito T, Wakamatsu Y, et al. Regionalized expression of the Dbx family homeobox genes in the embryonic CNS of the mouse. Mech. Dev. 56:25-39.
Background Reference 2:
Lacin H, Zhu Y, Wilson BA, et al. Dbx mediates neuronal specification and differentiation through cross-repressive, lineage-specific interactions with eve and hb9. Development 2009; 136:3257-66.
Background Reference 3:
Ma P, Zhao S, Zeng W, et al. Xenopus Dbx2 is involved in primary neurogenesis and early neural plate patterning. Biochem. Biophys. Res. Commun. 2011; 412:170-4.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6873

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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