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Western blot analysis in mouse NIH-3T3,Neuro-2a cell line and mouse stomach tissue and U251,A2058,A375,Sk-Br-3,T47D cell line lysates (35ug/lane).
Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in mouse NIH-3T3,Neuro-2a cell line and mouse stomach tissue and U251,A2058,A375,Sk-Br-3,T47D cell line lysates (35ug/lane).

DCBLD2 Antibody

CATALOG NUMBER: 57-298

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This DCBLD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 538-567 amino acids from the C-terminal region of human DCBLD2.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
85 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DCBLD2
Additional Names:
Discoidin, CUB and LCCL domain-containing protein 2, CUB, LCCL and coagulation factor V/VIII-homology domains protein 1, Endothelial and smooth muscle cell-derived neuropilin-like protein, DCBLD2, CLCP1, ESDN
Protein Accession Number:
Q96PD2
Protein Gi Number:
57013820
Ncbi Gene Id Number:
131566
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DCBLD2, otherwise known as ESDN (endothelial and smooth muscle cell-derived neuropilin-like molecule) is a novel type-I transmembrane protein with the longest cleavable secretory signal sequence among eukaryotes. It is expressed in various tissues; particularly highly expressed in cultured vascular smooth muscle cells. DCBLD2 is considered to play a role in regulation of vascular cell growth and may have a wide variety of functions in other tissues including the nervous system, like neuropilins.
Background Reference 1:
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Background Reference 2:
Guo, X., et al. J. Biol. Chem. 284(43):29376-29382(2009)
Background Reference 3:
Marroni, F., et al. Circ Cardiovasc Genet 2(4):322-328(2009)
Background Reference 4:
Kim, M., et al. Mol. Cancer Res. 6(2):222-230(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-298

List Size:
400 ul

List Price:

$495.00

Datasheet
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