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45-464 (0.2ug/ml) staining of COS7 cell lysate transfected with full length recombinant human DCDC2 (A) and untransfected control COS7 cells (B). Primary incubation was 1 hour. Detected by chemiluminescence.
45-464 (5ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-464 (0.2ug/ml) staining of COS7 cell lysate transfected with full length recombinant human DCDC2 (A) and untransfected control COS7 cells (B). Primary incubation was 1 hour. Detected by chemiluminescence.

DCDC2 Antibody

CATALOG NUMBER: 45-464

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig
Immunogen:
The immunogen for this antibody is: C-DGEKANKDAEQKED
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Full length recombinant human DCDC2 expressed in COS7 (Lane A), non-transfectant COS7 (lane B). Approx 52kDa band observed (calculated MW of 52.8kDa according to NP_057440.1). Data kindly provided by Wang and LoTurco, University of Connecticut, USA. Recommmended concentration 0.1-0.3ug/ml. An anonymous customer found positive results in WB on Mouse brain tissue.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows cytoplasm staining in select neurons. Recommended concentration, 5-10ug/ml. An anonymous customer found positive results in IHC on Rat retinal tissue. An anonymous customer found positive results in IHC on Rat dorsal root ganglion.
Positive Control 1:
COS7 Cell Lysate transfected
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DCDC2
Additional Names:
DCDC2, doublecortin domain containing 2, HGNC:18141, KIAA1154, RU2, RU2S, DCDC2A
Protein Accession Number:
NP_057440.2
Protein Gi Number:
7706691
Ncbi Gene Id Number:
51473
Background Reference 1:
Cope N, Harold D, Hill G, Moskvina V, Stevenson J, Holmans P, Owen MJ, O'Donovan MC, Williams J. Strong evidence that KIAA0319 on chromosome 6p is a susceptibility gene for developmental dyslexia. Am J Hum Genet. 2005 Apr;76(4):581-91. Epub 2005 Feb 16.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-464

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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