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Western Blot at 1:1000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of DCI Antibody in MCF-7, 293 cell line lysates (35ug/lane)
Western Blot at 1:1000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

DCI Antibody

CATALOG NUMBER: 63-848

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This DCI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human DCI.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
33 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ECI1
Additional Names:
Enoyl-CoA delta isomerase 1, mitochondrial,2-trans-enoyl-CoA isomerase, Delta(3),Delta(2)-enoyl-CoA isomerase, D3,D2-enoyl-CoA isomerase, Dodecenoyl-CoA isomerase, ECI1, DCI
Protein Accession Number:
P42126
Protein Gi Number:
1169204
Ncbi Gene Id Number:
1632
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DCI encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates.
Background Reference 1:
Partanen,S.T., et.al., J. Mol. Biol. 342 (4), 1197-1208 (2004)
Background Reference 2:
Wistow,G., et.al, Mol. Vis. 8, 171-184 (2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-848

List Size:
400 ul

List Price:

$495.00

Datasheet
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