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Western blot analysis of DCLK2 in rat brain tissue lysate with DCLK2 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of DLCK2 in rat brain tissue with DLCK2 antibody at 5 μg/mL.
Western blot analysis of DCLK2 in rat brain tissue lysate with DCLK2 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

DCLK2 Antibody

CATALOG NUMBER: 5635

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
DCLK2 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human DCLK2.
The immunogen is located within the last 50 amino acids of DCLK2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
DCLK2 antibody can be used for detection of DCLK2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in rat samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1413 – Mouse Cerebrum Tissue Lysate
Predicted Molecular Weight:
Predicted: 85 kDa
Observed: 85 kDa
Purification:
DCLK2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
DCLK2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
DCLK2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DCLK2
Additional Names:
DCLK2 Antibody: CL2, DCK2, CLIK2, DCDC3, CLICK2, DCDC3B, DCAMKL2, CLICK-II, CaMK-like CREB regulatory kinase 2, CL2
Protein Accession Number:
NP_001035350
Protein Gi Number:
156713428
Ncbi Gene Id Number:
166614
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DCLK2 Antibody: DCLK2 is one of three doublecortin-like kinases similar to the Ca2+/calmodulin-dependent protein kinase (CaMK) family. DCLK2 mRNA, like that of the homologous DCLK1 and DCLK3, is highly expressed in adult brain, but only DCLK1 and DCLK2 transcripts are present in human fetal brain and the developing mouse embryo, suggesting that DCLK1 and DCLK2 may play roles in cortical development. The DCLK proteins are homologous to Doublecortin (DCX), a protein that is mutated in X-linked human lissencephaly. In mouse models where the DCX gene has been disrupted, DCLK1 expression increases slightly and appears to compensate for the loss of DCX, as mice mutant for both DCX and DCLK1 show a severe phenotype including perinatal lethality, disorganized neocortical layering, and profound hippocampal cytoarchitectural disorganization. Unlike DCLK1, DCLK2 expression does not change in DCX-null mice.
Background Reference 1:
Sossey-Alaoui K and Srivastava AK. DCAMKL1, a brain specific transmembrane protein on 13q12.3 that is similar to doublecortin (DCX), Genomics1999; 56:121-6.
Background Reference 2:
Ohmae S, Takemoto-Kimura S, Okamura M, et al. Molecular identification and characterization of a family of kinases with homology to Ca2+/calmodulin-dependent protein kinases I/IV. J. Biol. Chem.2006; 281:20427-39.
Background Reference 3:
Tuy FPD, Saillour Y, Kappeler C, et al. Alternative transcripts of Dlck1 and Dlck2 and their expression in doublecortin knockout mice. Dev. Neurosci.2008; 30:171-86.
Background Reference 4:
Reiner O and Coquelle FM. Missense mutations resulting in type 1 lissencephaly. Cell Mol. Life Sci.2005; 62:425-34.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5635

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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