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Western blot analysis of DCN antibody in mouse heart tissue lysates (35ug/lane)
Western blot analysis of DCN using rabbit polyclonal DCN Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DCN gene.
Formalin-fixed and paraffin-embedded human hepatocarcinoma with DCN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of DCN antibody in mouse heart tissue lysates (35ug/lane)

DCN Antibody

CATALOG NUMBER: 62-350

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Pig
Immunogen:
This DCN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-150 amino acids from the Central region of human DCN.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
40 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit IgG
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DCN
Additional Names:
Decorin, Bone proteoglycan II, PG-S2, PG40, DCN, SLRR1B
Protein Accession Number:
P07585
Protein Gi Number:
129951
Ncbi Gene Id Number:
1634
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DCN is a small cellular or pericellular matrix proteoglycan that is closely related in structure to biglycan protein. The protein and biglycan are thought to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. It contains one attached glycosaminoglycan chain. This protein is capable of suppressing the growth of various tumor cell lines.
Background Reference 1:
Goldoni,S., J. Cell Biol. 185 (4), 743-754 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-350

List Size:
400 ul

List Price:

$495.00

Datasheet
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