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Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.

DCP2 Antibody

CATALOG NUMBER: 29-510

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DCP2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
DCP2 antibody can be used for detection of DCP2 by ELISA at 1:62500. DCP2 antibody can be used for detection of DCP2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
48 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store DCP2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
DCP2
Additional Names:
DCP2, FLJ33245, NUDT20
Protein Accession Number:
NP_689837
Protein Gi Number:
31542498
Ncbi Gene Id Number:
167227
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DCP2 is necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. DCP2 removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. It has higher activity towards mRNAs that lack a poly (A) tail, but has no activity towards a cap structure lacking a RNA moiety.DCP2 is a key component of an mRNA-decapping complex required for removal of the 5-prime cap from mRNA prior to its degradation from the 5-prime end (Fenger-Gron et al., 2005 [PubMed 16364915]).
Background Reference 1:
Yamochi, T., (2008) Biochem. Biophys. Res. Commun. 370 (1), 195-199.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-510

List Size:
100 ul

List Price:

$519.00

Datasheet
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