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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

DCPS Antibody

CATALOG NUMBER: 58-947

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig, Rat
Immunogen:
This DCPS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-193 amino acids from the Central region of human DCPS.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
39 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DCPS
Additional Names:
m7GpppX diphosphatase, DCS-1, Decapping scavenger enzyme, Hint-related 7meGMP-directed hydrolase, Histidine triad nucleotide-binding protein 5, Histidine triad protein member 5, HINT-5, Scavenger mRNA-decapping enzyme DcpS, DCPS, DCS1, HINT5
Protein Accession Number:
Q96C86
Protein Gi Number:
116241325
Ncbi Gene Id Number:
28960
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Necessary for the complete degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA fragments shorter than 10 nucleotides that are produced by 3'->5' exosome-mediated mRNA decay. Releases m7GMP. Can also degrade m7GDP to m7GMP. Has no activity towards mRNA molecules longer than 25 nucleotides.
Background Reference 1:
Sebastiani, P., et al. Science (2010) In press :
Background Reference 2:
Mariller, C., et al. Biochimie 91(1):109-122(2009)
Background Reference 3:
Liu, S.W., et al. J. Biol. Chem. 283(24):16427-16436(2008)
Background Reference 4:
Shen, V., et al. RNA 14(6):1132-1142(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-947

List Size:
400 ul

List Price:

$495.00

Datasheet
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