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Western blot analysis of Dcr-1 antibody in CEM cell line lysates (35ug/lane)
Western blot analysis of Dcr-1 antibody in CEM cell line lysates (35ug/lane)

Dcr-1 Antibody

CATALOG NUMBER: 60-630

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
C. elegans
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
C. elegans
Immunogen:
This Dcr-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1068-1097 amino acids from the Central region of human Dcr-1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
218 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
dcr-1
Additional Names:
Endoribonuclease dcr-1, 3126-, dcr-1
Protein Accession Number:
P34529
Ncbi Gene Id Number:
176138
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The Dcr-1 gene encodes a bidentate ribonuclease that is homologous to E. coli RNAse III. Dcr-1 is required both for RNA interference and for synthesis of small developmental RNAs. Dcr-1 interacts in vivo with RDE-4, a double-stranded RNA (dsRNA) binding protein required for RNAi that interacts with trigger dsRNAs and may function to deliver dsRNAs to Dcr-1 for endonucleolytic processing.
Background Reference 1:
Duchaine TF, et al. 2006. Cell 124:343-354.
Background Reference 2:
Denli AM, et al. 2004. Nature 432:231-235.
Background Reference 3:
Tabara H, et al. 2002. Cell 109:861-871.
Background Reference 4:
Grishok A, et al. 2001. Cell 106:23-34.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-630

List Size:
400 ul

List Price:

$495.00

Datasheet
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