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42-682 (0.03ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-682 (0.2ug/ml) staining of Mouse (A) and Rat (B) Skeletal Muscle lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-682 (0.03ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

DCTN3 Antibody

CATALOG NUMBER: 42-682

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-QVALGNISSKRE
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 20kDa band observed in Human Kidney, Skeletal Muscle and Pancreas lysates (calculated MW of 19.5kDa according to NP_077324.1). In addition, a 25kDa band is observed in both Mouse and Rat Skeletal Muscle lysates accompanied by an 18kDa band in Mouse Skeletal Msuscle. In Mouse the protein is known as p24. The 18kDa band could reprsent a smaller isoform. Recommended concentration: 0.03-0.1ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_009165.1; NP_077324.1).
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Positive Control 2:
Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate
Positive Control 3:
Cat. No. 1307 - Human Pancreas Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DCTN3
Additional Names:
DCTN3, dynactin 3 (p22), DCTN-22, DCTN22, MGC111190, dynactin 3, dynactin 3, isoform 1, dynactin light chain
Protein Accession Number:
NP_009165.1,NP_077324.1
Protein Gi Number:
6005745
Ncbi Gene Id Number:
11258

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-682

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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