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43-077 (0.5ug/ml) staining of Mouse fetal Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-077 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
43-077 (0.5ug/ml) staining of Mouse fetal Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Dcx Antibody

CATALOG NUMBER: 43-077

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: C-PKASPTPQKTSAKS
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 40kDa band observed in Mouse fetal Brain lysates (calculated MW of 40.6kDa according to NP_001103692.1). Recommended concentration: 0.5-2ug/ml. Primary incubation was 1 hour.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 5ug/ml
Specificity:
This antibody is expected to recognize all reported mouse isoforms (NP_001103692.1; NP_001103694.1; NP_034155.2). Reported variants represent identical protein: NP_001103692.1, NP_001103693.1
Positive Control 1:
Cat. No. 1403-0 - Mouse Brain Tissue Lysate (0 Day Old)
Predicted Molecular Weight:
Approx 40 kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Dcx
Additional Names:
Dcx, doublecortin, Dbct, OTTMUSP00000020394, OTTMUSP00000020395, OTTMUSP00000020396, OTTMUSP00000020397, doublecortex, doublin, lis-X, lissencephalin-X, lissencephaly, X-linked (doublecortin), neuronal migration protein doublecortin
Protein Accession Number:
NP_001103692.1,NP_001103694.1,NP_034155.2
Protein Gi Number:
158966719
Ncbi Gene Id Number:
1641

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-077

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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