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45-468 (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-468 (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

DDAH1 Antibody

CATALOG NUMBER: 45-468

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: TCCSVLINKKVDS
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 35kDa band observed in Human Kidney, Human Brain (Cerebellum) and Pancreas lysates (calculated MW of 31.1kDa according to NP_036269.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pullamsetti et al, FASEB J. 2005 Jul;19(9):1175-7; PMID: 15827267). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_036269.1and NP_001127917.1).
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DDAH1
Additional Names:
DDAH1, dimethylarginine dimethylaminohydrolase 1, DDAH, DDAHI, NG, NG-dimethylarginine dimethylaminohydrolase, RP4-621F18.1, FLJ21264, FLJ25539
Protein Accession Number:
NP_036269.1,NP_001127917.1
Protein Gi Number:
6912328
Ncbi Gene Id Number:
23576
Background Reference 1:
Kimoto M, Miyatake S, Sasagawa T, Yamashita H, Okita M, Oka T, Ogawa T, Tsuji H. Purification, cDNA cloning and expression of human NG, NG-dimethylarginine dimethylaminohydrolase. Eur J Biochem. 1998 Dec 1;258(2):863-8.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-468

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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