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45-469 (0.1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-469 (0.1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

DDAH2 Antibody

CATALOG NUMBER: 45-469

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: SSLCLVLSTRPHS
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 28kDa band observed in Human Lung lysates (calculated MW of 29.6kDa according to NP_039268.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.Immunofluorescence:Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10ug/ml.Flow Cytometry:Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DDAH2
Additional Names:
DDAH2, dimethylarginine dimethylaminohydrolase 2, DDAH, G6a, NG30, DDAHII, dimethylarginine dimethylaminohydrolase II, NG-dimethylarginine dimethylamino hydrolase homolog, OTTHUMP00000062666, DADB-110M10.5, OTTHUMP00000029307, OTTHUMP00000174406, alternative name: NG30, G6a, dimethylarginine dimethylaminohydrolase II
Protein Accession Number:
NP_039268.1
Protein Gi Number:
7524354
Ncbi Gene Id Number:
23564
Background Reference 1:
Leiper JM, Santa Maria J, Chubb A, MacAllister RJ, Charles IG, Whitley GS, Vallance P. Identification of two human dimethylarginine dimethylaminohydrolases with distinct tissue distributions and homology with microbial arginine deiminases. Biochem J 1999 Oct 1;343 Pt 1:209-14

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-469

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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