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45-048 (0.01ug/ml) staining of NIH3T3 (A) and NSO (B) lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-048 (1ug/ml) staining of HeLa (A), HepG2 (B) and Jurkat (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-048 (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-048 (0.01ug/ml) staining of NIH3T3 (A) and NSO (B) lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence.

DDB1 Antibody

CATALOG NUMBER: 45-048

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-DLIKVVEELTRIH
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 140kDa band observed in lysates of cell line HeLa, HepG2 and Jurkat (calculated MW of 126.9kDa according to Human NP_001914.3). Recommended concentration: 1-2ug/ml. Approx 140kDa band observed in lysates of cell line NIH3T3 and approx 150kDa band observed in lysates of cell line NSO (calculated MW of 126.8kDa according to Mouse NP_056550.1). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Kidney for this particular batch.
Positive Control 1:
NSO Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DDB1
Additional Names:
DDB1, damage-specific DNA binding protein 1, 127kDa, DDBA, XAP1, XPCE, XPE-BF, UV-DDB1, DDB p127 subunit, damage-specific DNA binding protein 1 (127kD), XPE
Protein Accession Number:
NP_001914.3
Protein Gi Number:
148529014
Ncbi Gene Id Number:
1642
Background Reference 1:
Dualan R, Brody T, Keeney S, Nichols AF, Admon A, Linn S. Chromosomal localization and cDNA cloning of the genes (DDB1 and DDB2) for the p127 and p48 subunits of a human damage-specific DNA binding protein. Genomics 1995 Sep 1;29(1):62-9

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-048

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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