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Antibody used in WB on Human Jurkat 5.0 ug/ml.
Antibody used in WB on Human Jurkat 5.0 ug/ml.

DDOST Antibody

CATALOG NUMBER: 30-207

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat, Zebrafish
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat, Zebrafish
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DDOST.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
DDOST antibody can be used for detection of DDOST by ELISA at 1:62500. DDOST antibody can be used for detection of DDOST by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
46 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store DDOST antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
DDOST
Additional Names:
DDOST, AGE-R1, KIAA0115, MGC2191, OK/SW-cl.45, OST, OST48, WBP1, AGER1, CDG1R, OKSWcl45
Protein Accession Number:
NP_005207
Protein Gi Number:
20070197
Ncbi Gene Id Number:
1650
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DDOST is a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. DDOST is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia.
Background Reference 1:
Shibatani, T., (2005) Biochemistry 44 (16), 5982-5992.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-207

List Size:
100 ul

List Price:

$473.00

Datasheet
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