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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.

DDX11 Antibody

CATALOG NUMBER: 55-911

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This DDX11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 819-847 amino acids from the C-terminal region of human DDX11.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
108 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DDX11
Additional Names:
Probable ATP-dependent RNA helicase DDX11, CHL1-related protein 1, hCHLR1, DEAD/H box protein 11, Keratinocyte growth factor-regulated gene 2 protein, KRG-2, DDX11, CHL1, CHLR1, KRG2
Protein Accession Number:
Q96FC9
Protein Gi Number:
74731686
Ncbi Gene Id Number:
1663
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DEAD box proteins, characterized by the conserved motifAsp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They areimplicated in a number of cellular processes involving alterationof RNA secondary structure such as translation initiation, nuclearand mitochondrial splicing, and ribosome and spliceosome assembly.Based on their distribution patterns, some members of this familyare believed to be involved in embryogenesis, spermatogenesis, andcellular growth and division. This gene encodes a DEAD box protein,which is an enzyme that possesses both ATPase and DNA helicaseactivities. This gene is a homolog of the yeast CHL1 gene, and mayfunction to maintain chromosome transmission fidelity and genomestability. Alternative splicing results in multiple transcriptvariants encoding distinct isoforms.
Background Reference 1:
Leman, A.R., et al. J. Cell. Sci. 123 (PT 5), 660-670 (2010) :
Background Reference 2:
Farina, A., et al. J. Biol. Chem. 283(30):20925-20936(2008)
Background Reference 3:
Parish, J.L., et al. Mol. Cell 24(6):867-876(2006)
Background Reference 4:
Parish, J.L., et al. J. Cell. Sci. 119 (PT 23), 4857-4865 (2006) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-911

List Size:
400 ul

List Price:

$495.00

Datasheet
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