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Western blot analysis of DDX17 Antibody in NCI-H460 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with DDX17 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of DDX17 Antibody in NCI-H460 cell line lysates (35ug/lane)

DDX17 Antibody

CATALOG NUMBER: 63-895

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This DDX17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 128-155 amino acids from the N-terminal region of human DDX17.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
80 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DDX17
Additional Names:
Probable ATP-dependent RNA helicase DDX17, DEAD box protein 17, DEAD box protein p72, RNA-dependent helicase p72, DDX17
Protein Accession Number:
Q92841
Ncbi Gene Id Number:
10521
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DDX17 box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and splicesosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This protein encodes a DEAD box protein, which is an ATPase activated by a variety of RNA species, but not by dsDNA. This protein, and that encoded by DDX5 gene, are more closely related to each other than to any other member of the DEAD box family.
Background Reference 1:
Wortham,N.C., et.al., Oncogene 28 (46), 4053-4064 (2009)
Background Reference 2:
Tonevitsky,E.A., et.al., Bull. Exp. Biol. Med. 147 (6), 733-736 (2009)
Background Reference 3:
Wong,H.Y., et.al., Biochim. Biophys. Acta 1794 (2), 193-198 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-895

List Size:
400 ul

List Price:

$495.00

Datasheet
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