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Western Blot at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: HT-1080 whole cell lysate Lane 6: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: HT-1080 whole cell lysate Lane 6: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.

DDX21 Antibody

CATALOG NUMBER: 59-054

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This DDX21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 128-156 amino acids from the N-terminal region of human DDX21.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:2000
Predicted Molecular Weight:
87 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DDX21
Additional Names:
Nucleolar RNA helicase 2, DEAD box protein 21, Gu-alpha, Nucleolar RNA helicase Gu, Nucleolar RNA helicase II, RH II/Gu, DDX21
Protein Accession Number:
Q9NR30
Protein Gi Number:
76803555
Ncbi Gene Id Number:
9188
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DEAD box proteins, characterized by the conserved motifAsp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They areimplicated in a number of cellular processes involving alterationof RNA secondary structure such as translation initiation, nuclearand mitochondrial splicing, and ribosome and spliceosome assembly.Based on their distribution patterns, some members of this familyare believed to be involved in embryogenesis, spermatogenesis, andcellular growth and division. This gene encodes a DEAD box protein,which is an antigen recognized by autoimmune antibodies from apatient with watermelon stomach disease. This protein unwindsdouble-stranded RNA, folds single-stranded RNA, and may playimportant roles in ribosomal RNA biogenesis, RNA editing, RNAtransport, and general transcription.
Background Reference 1:
Holmstrom, T.H., et al. J. Biol. Chem. 283(11):7046-7053(2008)
Background Reference 2:
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Background Reference 3:
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Background Reference 4:
Olsen, J.V., et al. Cell 127(3):635-648(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-054

List Size:
400 ul

List Price:

$495.00

Datasheet
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