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Western blot analysis of DDX23 Antibody in 293 cell line lysates (35ug/lane)
DDX23 Antibody IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of DDX23 Antibody in 293 cell line lysates (35ug/lane)

DDX23 Antibody

CATALOG NUMBER: 61-690

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This DDX23 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 248-277 amino acids from the Central region of human DDX23.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
96 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DDX23
Additional Names:
Probable ATP-dependent RNA helicase DDX23, 100 kDa U5 snRNP-specific protein, DEAD box protein 23, PRP28 homolog, U5-100kD, DDX23
Protein Accession Number:
Q9BUQ8
Protein Gi Number:
160385708
Ncbi Gene Id Number:
9416
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DDX23 encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein is a component of the U5 snRNP complex; it may facilitate conformational changes in the spliceosome during nuclear pre-mRNA splicing.
Background Reference 1:
Mathew, R., et al. Nat. Struct. Mol. Biol. 15(5):435-443(2008)
Background Reference 2:
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007)
Background Reference 3:
Olsen, J.V., et al. Cell 127(3):635-648(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-690

List Size:
400 ul

List Price:

$495.00

Datasheet
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