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Western blot analysis in A549 cell line lysates (35ug/lane).
Western blot analysis in A549 cell line lysates (35ug/lane).

DDX28 Antibody

CATALOG NUMBER: 58-493

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This DDX28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 370-398 amino acids from the C-terminal region of human DDX28.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
60 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DDX28
Additional Names:
Probable ATP-dependent RNA helicase DDX28, Mitochondrial DEAD box protein 28, DDX28, MDDX28
Protein Accession Number:
Q9NUL7
Protein Gi Number:
296434476
Ncbi Gene Id Number:
55794
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DEAD box proteins, characterized by the conserved motifAsp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They areimplicated in a number of cellular processes involving alterationof RNA secondary structure, such as translation initiation, nuclearand mitochondrial splicing, and ribosome and spliceosome assembly.Based on their distribution patterns, some members of the DEAD boxprotein family are believed to be involved in embryogenesis,spermatogenesis, and cellular growth and division. This gene isintronless. It encodes an RNA-dependent ATPase. The encoded proteinis localized in the mitochondria and the nucleus, and can betransported between the mitochondria and the nucleus. [provided byRefSeq].
Background Reference 1:
Bogenhagen, D.F., et al. J. Biol. Chem. 283(6):3665-3675(2008)
Background Reference 2:
Valgardsdottir, R., et al. J. Biol. Chem. 278(23):21146-21154(2003)
Background Reference 3:
Valgardsdottir, R., et al. J. Biol. Chem. 276(34):32056-32063(2001)
Background Reference 4:
Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-493

List Size:
400 ul

List Price:

$495.00

Datasheet
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