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Western blot analysis in WiDr cell line lysates (35ug/lane).
Western blot analysis in mouse testis tissue lysates (35ug/lane).
Western blot analysis in WiDr cell line lysates (35ug/lane).

DDX4 Antibody

CATALOG NUMBER: 59-092

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This DDX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-131 amino acids from the N-terminal region of human DDX4.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
79 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DDX4
Additional Names:
Probable ATP-dependent RNA helicase DDX4, DEAD box protein 4, Vasa homolog, DDX4, VASA
Protein Accession Number:
Q9NQI0
Protein Gi Number:
20138033
Ncbi Gene Id Number:
54514
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DEAD box proteins, characterized by the conserved motifAsp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They areimplicated in a number of cellular processes involving alterationof RNA secondary structure such as translation initiation, nuclearand mitochondrial splicing, and ribosome and spliceosome assembly.Based on their distribution patterns, some members of this familyare believed to be involved in embryogenesis, spermatogenesis, andcellular growth and division. This gene encodes a DEAD box protein,which is a homolog of VASA proteins in Drosophila and several otherspecies. The gene is specifically expressed in the germ celllineage in both sexes and functions in germ cell development.Multiple transcript variants encoding different isoforms have beenfound for this gene.
Background Reference 1:
Tilgner, K., et al. Stem Cells 28(1):84-92(2010)
Background Reference 2:
Sugimoto, K., et al. J. Hum. Genet. 54(8):450-456(2009)
Background Reference 3:
Hashimoto, H., et al. Gynecol. Oncol. 111(2):312-319(2008)
Background Reference 4:
Albamonte, M.S., et al. Hum. Reprod. 23(8):1895-1901(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-092

List Size:
400 ul

List Price:

$495.00

Datasheet
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