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Antibody used in WB on Human Jurkat 2.5 ug/ml.
Antibody used in WB on Human Jurkat 2.5 ug/ml.

DDX47 Antibody

CATALOG NUMBER: 28-494

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DDX47.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
DDX47 antibody can be used for detection of DDX47 by ELISA at 1:1562500. DDX47 antibody can be used for detection of DDX47 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
50 kDa, 45 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store DDX47 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
DDX47
Additional Names:
DDX47, RRP3, E4-DBP, HQ0256, MSTP162
Protein Accession Number:
NP_057439
Protein Gi Number:
20149629
Ncbi Gene Id Number:
51202
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DDX47 encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene can shuttle between the nucleus and the cytoplasm, and has an RNA-independent ATPase activity. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Background Reference 1:
Doorbar, J., et al., (2000) J. Virol. 74 (21), 10081-10095.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-494

List Size:
100 ul

List Price:

$473.00

Datasheet
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