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45-470 (0.5ug/ml) staining of cell lines A431(A), HeLa(B), HepG2(C), Jurkat(D) and NIH3T3 (E) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-470 (2.5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-470 (2.5ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-470 (2.5ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-470 (0.5ug/ml) staining of cell lines A431(A), HeLa(B), HepG2(C), Jurkat(D) and NIH3T3 (E) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

DDX5 Antibody

CATALOG NUMBER: 45-470

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog
Immunogen:
The immunogen for this antibody is: C-PMIGYPMPTGYSQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 70kDa band observed in nuclear lysates of cell lines NIH3T3, A431, HeLa and Jurkat (calculated MW of 69.1kDa according to NP_004387.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunofluorescence: A customer has reported successful staining at a dilution of 1:100 on HT-1080 cells with a Rhodamine conjugated secondary antibody. This data is from a previous batch, not on sale.
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DDX5
Additional Names:
DDX5, P68, HLR1, G17P1, HUMP68, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kDa), RNA-dependent ATPase, DEAD/H box-5 (RNA helicase, 68kD), DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD), DEAD (Asp-Glu-Ala-Asp) box polypeptide 5, DKFZp434E109, DKFZp686J01190, ATP-dependent RNA helicase DDX5, DEAD box-5
Protein Accession Number:
NP_004387.1
Protein Gi Number:
4758138
Ncbi Gene Id Number:
1655
Background Reference 1:
Iggo R, Gough A, Xu W, Lane DP, Spurr NK. Chromosome mapping of the human gene encoding the 68- kDa nuclear antigen (p68) by using the polymerase chain reaction. Proc Natl Acad Sci USA. 1989 Aug;86(16):6211-4.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-470

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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