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Western blot analysis of DDX5 antibody in Romas cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain with DDX5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of DDX5 antibody in Romas cell line lysates (35ug/lane)

DDX5 Antibody

CATALOG NUMBER: 62-931

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey, Mouse
Immunogen:
This DDX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 306-334 amino acids from the Central region of human DDX5.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
69 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DDX5
Additional Names:
Probable ATP-dependent RNA helicase DDX5, DEAD box protein 5, RNA helicase p68, DDX5, G17P1, HELR, HLR1
Protein Accession Number:
P17844
Protein Gi Number:
129383
Ncbi Gene Id Number:
1655
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DDX5 is putative RNA helicases. The protein is implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This protein is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen.
Background Reference 1:
Ong S.E., Mittler G.Nat. Methods 1:119-126(2004)
Background Reference 2:
Daub H., Olsen J.V.Mol. Cell 31:438-448(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-931

List Size:
400 ul

List Price:

$495.00

Datasheet
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