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Western blot analysis of DDX53 antibody in mouse kidney tissue lysates (35ug/lane)
Flow cytometric analysis of WiDr cells using DDX53 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of DDX53 antibody in mouse kidney tissue lysates (35ug/lane)

DDX53 Antibody

CATALOG NUMBER: 62-946

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This DDX53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 597-626 amino acids from the C-terminal region of human DDX53.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
71 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DDX53
Additional Names:
Probable ATP-dependent RNA helicase DDX53, Cancer-associated gene protein, Cancer/testis antigen 26, CT26, DEAD box protein 53, DEAD box protein CAGE, DDX53, CAGE
Protein Accession Number:
Q86TM3
Protein Gi Number:
143811384
Ncbi Gene Id Number:
168400
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background Reference 1:
Shim,H., Lee,H. Biotechnol. Lett. 28 (24), 2057-2063 (2006)
Background Reference 2:
Cho,B., Lee,H. Biochem. Biophys. Res. Commun. 307 (1), 52-63 (2003)
Background Reference 3:
Cho,B., Lim,Y. Biochem. Biophys. Res. Commun. 292 (3), 715-726 (2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-946

List Size:
400 ul

List Price:

$495.00

Datasheet
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