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Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.

DDX55 Antibody

CATALOG NUMBER: 28-499

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DDX55.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
DDX55 antibody can be used for detection of DDX55 by ELISA at 1:312500. DDX55 antibody can be used for detection of DDX55 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
66 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store DDX55 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
DDX55
Additional Names:
DDX55, KIAA1595
Protein Accession Number:
NP_065987
Protein Gi Number:
41327779
Ncbi Gene Id Number:
57696
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DDX55 is a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of only one transcript has been confirmed.
Background Reference 1:
Schmid, S.R., et al., (1992) Mol. Microbiol. 6 (3), 283-291.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-499

List Size:
100 ul

List Price:

$519.00

Datasheet
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