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Western blot analysis in Hela,MCF-7 cell line lysates (35ug/lane).
DDX6 Antibody IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in Hela,MCF-7 cell line lysates (35ug/lane).

DDX6 Antibody

CATALOG NUMBER: 64-175

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Mouse, Xenopus
Immunogen:
This DDX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 348-375 amino acids from the Central region of human DDX6.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
54 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DDX6
Additional Names:
Probable ATP-dependent RNA helicase DDX6, ATP-dependent RNA helicase p54, DEAD box protein 6, Oncogene RCK, DDX6, HLR2, RCK
Protein Accession Number:
P26196
Protein Gi Number:
116241327
Ncbi Gene Id Number:
1656
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing.
Background Reference 1:
Broytman, O., et al. Neurobiol. Aging 30(12):1962-1974(2009)
Background Reference 2:
Han, J.W., et al. Nat. Genet. 41(11):1234-1237(2009)
Background Reference 3:
Minshall, N., et al. Mol. Biol. Cell 20(9):2464-2472(2009)
Background Reference 4:
Tritschler, F., et al. Mol. Cell 33(5):661-668(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-175

List Size:
400 ul

List Price:

$495.00

Datasheet
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