Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

42-495 (2ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer).Detected by chemiluminescence.
42-495 (3.8ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-495 (2ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer).Detected by chemiluminescence.

DEFA1/3 Antibody

CATALOG NUMBER: 42-495

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-DESLAPKHPGSRKN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 10kDa band observed in Human Spleen lysates (calculated MW of 10.2kDa according to NP_004075.1). . An additional band of unknown identity was also consistently observed at 20kDa, and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3ug/ml. . Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Thymus. Recommended concentration: 3-6ug/ml.
Specificity:
This antibody is expected to equally recognize DEFA1 precursor and DEFA3 precursor.
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DEFA1/3
Additional Names:
DEFA1, defensin, alpha 1, myeloid-related sequence , HGNC:2761, DEF1, DEFA2, HNP-1, HP-1, MRS , defensin, alpha 1, defensin, alpha 2, myeloid-related sequence, DEFA3, defensin, alpha 3, neutrophil-specific, DEF3, HNP-3, HNP3, HP-3, defensin 3, neutrophil-specific, defensin, alpha 3, neutrophil peptide 3
Protein Accession Number:
NP_004075.1,NP_005208.1
Protein Gi Number:
4758146
Ncbi Gene Id Number:
1667;1668

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-495

List Size:
0.1 mg

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369