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Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.

DEK Antibody

CATALOG NUMBER: 27-236

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DEK.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
DEK antibody can be used for detection of DEK by ELISA at 1:1562500. DEK antibody can be used for detection of DEK by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1219 - MCF7 Cell Lysate
Predicted Molecular Weight:
43 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store DEK antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
DEK
Additional Names:
DEK, D6S231E
Protein Accession Number:
NP_003463
Protein Gi Number:
4503249
Ncbi Gene Id Number:
7913
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DEK is a protein with one SAP domain. This protein binds to cruciform and superhelical DNA and induces positive supercoils into closed circular DNA, and is also involved in splice site selection during mRNA processing. Chromosomal aberrations involving this region, increased expression of this gene, and the presence of antibodies against this protein are all associated with various diseases. Two transcript variants encoding different isoforms have been found for this gene.This gene encodes a protein with one SAP domain. This protein binds to cruciform and superhelical DNA and induces positive supercoils into closed circular DNA and is also involved in splice site selection during mRNA processing. Chromosomal aberrations involving this region, increased expression of this gene and the presence of antibodies against this protein are all associated with various diseases. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Background Reference 1:
Wu, Q., (2008) Pathol. Int. 58 (6), 378-382.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-236

List Size:
100 ul

List Price:

$519.00

Datasheet
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