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Western blot analysis of lysates from U-251 MG, PC-3 cell line (from left to right), using DEK Antibody at 1:1000 at each lane.
DEK Antibody IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of DEK Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of lysates from U-251 MG, PC-3 cell line (from left to right), using DEK Antibody at 1:1000 at each lane.

DEK Antibody

CATALOG NUMBER: 63-827

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This DEK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 336-364 amino acids from the C-terminal region of human DEK.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
43 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DEK
Additional Names:
Protein DEK, DEK
Protein Accession Number:
P35659
Protein Gi Number:
544150
Ncbi Gene Id Number:
7913
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DEK is a protein with one SAP domain. This protein binds to cruciform and superhelical DNA and induces positive supercoils into closed circular DNA, and is also involved in splice site selection during mRNA processing. Chromosomal aberrations involving this region, increased expression of this gene, and the presence of antibodies against this protein are all associated with various diseases.
Background Reference 1:
Sawatsubashi,S., et.al., Genes Dev. 24 (2), 159-170 (2010)
Background Reference 2:
Khodadoust,M.S., et.al., Cancer Res. 69 (16), 6405-6413 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-827

List Size:
400 ul

List Price:

$495.00

Datasheet
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