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Western Blot at 1:500 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
DENND1B antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:500 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.

DENND1B Antibody

CATALOG NUMBER: 55-155

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This DENND1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 amino acids from the N-terminal region of human DENND1B.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:500
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
48 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DENND1B
Additional Names:
DENN domain-containing protein 1B, Connecdenn 2, Protein FAM31B, DENND1B, C1orf218, FAM31B
Protein Accession Number:
Q6P3S1
Protein Gi Number:
74749089
Ncbi Gene Id Number:
163486
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Clathrin (see MIM 118955)-mediated endocytosis is a majormechanism for internalization of proteins and lipids. Members ofthe connecdenn family, such as DENND1B, function as guaninenucleotide exchange factors (GEFs) for the early endosomal smallGTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptorprotein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35activation with the clathrin machinery (Marat and McPherson, 2010[PubMed 20154091]).
Background Reference 1:
Marat, A.L., et al. J. Biol. Chem. 285(14):10627-10637(2010)
Background Reference 2:
Sleiman, P.M., et al. N. Engl. J. Med. 362(1):36-44(2010)
Background Reference 3:
Venter, J.C., et al. Science 291(5507):1304-1351(2001)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-155

List Size:
400 ul

List Price:

$495.00

Datasheet
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