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Western blot analysis of DEPTOR in mouse liver tissue lysate with DEPTOR antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of DEPTOR in mouse liver tissue with DEPTOR antibody at 5 μg/mL.
Western blot analysis of DEPTOR in mouse liver tissue lysate with DEPTOR antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

DEPTOR Antibody

CATALOG NUMBER: 7787

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
DEPTOR antibody was raised against a 16 amino acid peptide near the center of human DEPTOR.
The immunogen is located within amino acids 220 - 270 of DEPTOR.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
DEPTOR antibody can be used for detection of DEPTOR by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Specificity:
DEPTOR antibody is human, mouse and rat reactive. At least three isoforms of DEPTOR are known to exist; this antibody will detect all three isoforms.
Positive Control 1:
Cat. No. 1404 - Mouse Liver Tissue Lysate
Predicted Molecular Weight:
Predicted: 45 kDa
Observed: 42 kDa
Purification:
DEPTOR antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
DEPTOR Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
DEPTOR antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
DEPTOR
Additional Names:
DEPTOR Antibody: DEP.6, DEPDC6, DEP domain-containing mTOR-interacting protein, DEP domain-containing protein 6
Protein Accession Number:
NP_073620
Protein Gi Number:
189571664
Ncbi Gene Id Number:
64798
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DEPTOR is an mTOR (mammalian target of rapamycin)-interacting protein whose expression is negatively regualted by mTOR complex 1 (mTORC1) and mTORC2 (1). Loss of DEPTOR activates RP6SK1, Akt1, and SGK1, promotes cell growth and survival, and activates mTORC1 and mTORC2 kinase activities. DEPTOR expression is low in most cancers, but is often elevated in multiple myelomas (1). In respons to stimulation by mitogens, DEPTOR is rapidly phosphorylated leading to its ubiquitination and degradation, allowing mTOR to generate an auto-amplification loop to promotes its own full activation (2).
Background Reference 1:
Peterson TR, Laplante M, Thoreen CC, et al. DEPTOR is an mTOR inhibitor frequently overexpressed in multiple myeloma cells and required for survival. Cell 2009; 137:873-6.
Background Reference 2:
Duan S, Skaar JR, Kuchay S, et al. mTOR generates an auto-amplification loop by triggering the bTrCP- and CK1a-dependent degradation of DEPTOR. Mol. Cell 2011; 44:317-24.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7787

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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