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46-777 (1ug/ml) staining of Human (A), Mouse (B), Rat (C) and Pig (D) Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-777 (5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Data obtained from previous batch.
46-777 (5ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-777 (3.75ug/ml) staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
46-777 Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody.
46-777 (1ug/ml) staining of Human (A), Mouse (B), Rat (C) and Pig (D) Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Desmin Antibody

CATALOG NUMBER: 46-777

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-RDGEVVSEATQQQHE
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 55-60kDa band observed in Human, Mouse, Rat and Pig Skeletal Muscle lysates (calculated MW of 53.5kDa according to Human NP_001918.3, Mouse NP_034173.1, Rat NP_071976.1 and 53.6kDa according to Pig NP_001001535.1). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Heart and Skeletal Muscle. Recommended concentration: 5ug/ml. In paraffin embedded Human Heart, expression of Desmin is detected in myocardial muscle and not in adjacent vascular smooth muscle. Recommended concentration:3.75ug/ml. Data obtained from previous batch.
Positive Control 1:
Cat. No. 1461 - Rat Heart Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DES
Additional Names:
DES, desmin, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, intermediate filament protein
Protein Accession Number:
NP_001918.3
Protein Gi Number:
55749932
Ncbi Gene Id Number:
1674
Background Reference 1:
Chen F, Chang R, Trivedi M, Capetanaki Y, Cryns VL. Caspase proteolysis of desmin produces a dominant-negative inhibitor of intermediate filaments and promotes apoptosis. J Biol Chem. 2003 Feb 28;278(9):6848-53.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-777

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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