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42-180 (3.8ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-180 (3.8ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Desmoglein 1 Antibody

CATALOG NUMBER: 42-180

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-YTTSDTLKPSVHVHD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments in lysates of cell lines A431, HeLa, K562 and MOLT4 gave no specific signal but low background (at antibody concentration up to 1ug/ml).Immunohistochemistry:In paraffin embedded Human Skin showsstaining of the cellular membranes of keratinocytes. Recommended concentration, 4-6ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DSG1
Additional Names:
DSG1, desmoglein 1, CDHF4, DG1, DSG, desmosomal glycoprotein 1, pemphigus foliaceus antigen
Protein Accession Number:
NP_001933.2
Protein Gi Number:
119703744
Ncbi Gene Id Number:
1828
Background Reference 1:
Nilles L.A., Parry D.A., Powers E.E., Angst B.D., Wagner R.M., Green K.J Structural analysis and expression of human desmoglein: a cadherin-like component of the desmosome J. Cell Sci. 1991 Aug 99 ( Pt 4): 809-21

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-180

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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