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IHC testing of FFPE human skin with Desmoglein 3 antibody (clone DSG3/1535). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
IHC testing of FFPE human lung squamos cell carcinoma with Desmoglein 3 antibody (clone DSG3/1535). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
IHC testing of FFPE human skin with Desmoglein 3 antibody (clone DSG3/1535). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Desmoglein 3 Antibody [DSG3/1535]

CATALOG NUMBER: 33-689

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
A protein fragment corresponding to the extracellular portion of human DSG3 was used as the immunogen for the Desmoglein 3 antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
WB: 0.5-1 ug/ml
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT
The stated application concentrations are suggested starting amounts. Titration of the Desmoglein 3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
DSG3
Ncbi Gene Id Number:
1830
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Desmoglein 3 is a component of intercellular desmosome junctions. It is involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. [UniProt]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-689

List Size:
100 ug

List Price:

$626.00

Datasheet
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