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Western blot analysis of DFNA5 Antibody in Hela cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with DFNA5 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of MDA-MB468 cells using DFNA5 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of DFNA5 Antibody in Hela cell line lysates (35ug/lane)

DFNA5 Antibody

CATALOG NUMBER: 62-495

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This DFNA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-87 amino acids from the N-terminal region of human DFNA5.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
55 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DFNA5
Additional Names:
Non-syndromic hearing impairment protein 5, Inversely correlated with estrogen receptor expression 1, ICERE-1, DFNA5, ICERE1
Protein Accession Number:
O60443
Ncbi Gene Id Number:
1687
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Hearing impairment is a heterogeneous condition with over 40 loci described. DFNA5 is expressed in fetal cochlea, however, its function is not known.
Background Reference 1:
Cheng,J., et.al., Clin. Genet. 72 (5), 471-477 (2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-495

List Size:
200 ul

List Price:

$495.00

Datasheet
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