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45-475 (0.3ug/ml) staining of Rat Duodenum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-475 (3.75ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-475 (0.3ug/ml) staining of Rat Duodenum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

DGAT1 Antibody

CATALOG NUMBER: 45-475

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Rat
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog
Immunogen:
The immunogen for this antibody is: C-QNSMKPFKDMDYS
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 55kDa band observed in Rat Duodenum lysates (calculated MW of 55.3kDa according to NP_036211.1). Recommended concentration: 0.3-1.0ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Colon. Recommended concentration: 3.75ug/ml.
Positive Control 1:
Cat. No. 1364 - Human Duodenum Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DGAT1
Additional Names:
DGAT1, diacylglycerol O-acyltransferase homolog 1 (mouse) , ARGP1, DGAT , ACAT related gene product 1, acyl coenzyme A:cholesterol acyltransferase related gene 1, diacylglycerol O-acyltransferase 1, diglyceride acyltransferase
Protein Accession Number:
NP_036211.1
Protein Gi Number:
6912332
Ncbi Gene Id Number:
8694
Background Reference 1:
Bagnato C, Igal RA. Overexpression of diacylglycerol acyltransferase-1 reduces phospholipid synthesis, proliferation, and invasiveness in simian virus 40-transformed human lung fibroblasts. J Biol Chem. 2003 Dec 26;278(52):52203-11. Epub 2003 Oct 13.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-475

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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