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45-476 (3ug/ml) staining of paraffin embedded Human Liver. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
45-476 (0.5ug/ml) as the reporter antibody.
45-476 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
45-476 (3ug/ml) staining of paraffin embedded Human Liver. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

DGAT2 Antibody

CATALOG NUMBER: 45-476

Clonality:
Polyclonal
Tested Applications:
EIA, ELISA, Flow, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-KLEHPTQQDIDLYH
Conjugate:
Unconjugated
Tested Applications:
EIA, ELISA, Flow, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Immunohistochemistry:In paraffin embedded Human Liver shows granular cytoplasm staining of hepatocytes. Recommended concentration: 3ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant DGAT2 captured by a rabbit antibody. Recommended reporter concentration: 0.5-1ug/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Specificity:
This antibody is not expected to cross-react with other DGAT members.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Positive Control 2:
Cat. No. 10-201 - Human Liver Tissue Slide
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DGAT2
Additional Names:
DGAT2, diacylglycerol O-acyltransferase homolog 2 (mouse), DKFZp686A15125, HMFN1045 , GS1999full, diacylglycerol O-acyltransferase homolog 2, diacylglycerol O-acyltransferase-like protein 2 isoform 1
Protein Accession Number:
NP_115953.2,NP_001240820.1
Protein Gi Number:
26024197
Ncbi Gene Id Number:
84649
Background Reference 1:
Cases S, Stone SJ, Zhou P, Yen E, Tow B, Lardizabal KD, Voelker T, Farese RV Jr. Cloning of DGAT2, a second mammalian diacylglycerol acyltransferase, and related family members. J Biol Chem. 2001 Oct 19;276(42):38870-6. Epub 2001 Jul 31.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-476

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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