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Western blot analysis of anti-hDGKB-T787 Pab in mouse liver tissue lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis in HepG2 cell line lysates (35ug/lane).
Western blot analysis of anti-hDGKB-T787 Pab in mouse liver tissue lysate.

DGKB Antibody

CATALOG NUMBER: 63-408

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This DGKB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 773-804 amino acids from the C-terminal region of human DGKB.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
91 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DGKB
Additional Names:
Diacylglycerol kinase beta, DAG kinase beta, 90 kDa diacylglycerol kinase, Diglyceride kinase beta, DGK-beta, DGKB, DAGK2, KIAA0718
Protein Accession Number:
Q9Y6T7
Protein Gi Number:
12643960
Ncbi Gene Id Number:
1607
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Diacylglycerol (DAG) is an allosteric activator of protein kinase C. DAG also participates in regulating RAS and RHO family proteins by activating the guanine nucleotide exchange factors VAV and RASGRP1. DAG is also involved in the synthesis of phospholipids and triacylglycerols. Tight regulation of DAG levels is achieved via DAG kinases (DGKs), which remove DAG by phosphorylate it to phosphatidic acid. Several mammalian isozymes of DAGK have been identified
Background Reference 1:
Caricasole, A., et al., J. Biol. Chem. 277(7):4790-4796 (2002).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-408

List Size:
400 ul

List Price:

$495.00

Datasheet
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