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Antibody used in WB on Human brain at 0.2-1 ug/ml.
Antibody used in WB on Mouse at 1:1000.
Antibody used in WB on Human brain at 0.2-1 ug/ml.

DGKE Antibody

CATALOG NUMBER: 26-054

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DGKE.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
DGKE antibody can be used for detection of DGKE by ELISA at 1:1562500. DGKE antibody can be used for detection of DGKE by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10123 - Fetal Brain Tissue Lysate
Predicted Molecular Weight:
64 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store DGKE antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
DGKE
Additional Names:
DGKE, DAGK6, DGK, DAGK5, NPHS7
Protein Accession Number:
NP_003638
Protein Gi Number:
4503313
Ncbi Gene Id Number:
8526
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis.Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis.
Background Reference 1:
Epand, R.M., (2007) Biochemistry 46 (49), 14225-14231.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

26-054

List Size:
100 ul

List Price:

$519.00

Datasheet
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