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Western blot analysis of extracts of various cell lines, using DGKQ antibody (19-175) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human breast cancer using DGKQ antibody (19-175) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using DGKQ antibody (19-175) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

DGKQ Antibody

CATALOG NUMBER: 19-175

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 683-942 of human DGKQ (NP_001338.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:200 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
K-562
Positive Control 3:
MCF-7
Positive Control 4:
Mouse brain
Positive Control 5:
Mouse kidney
Predicted Molecular Weight:
Observed: 101kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
DGKQ
Additional Names:
DAG kinase theta, DAGK, DAGK4, DAGK7, DGKQ, DGK-theta, diacylglycerol kinase theta, diacylglycerol kinase, theta 110kDa, diglyceride kinase theta, OTTHUMP00000112179, OTTHUMP00000217323
Ncbi Gene Id Number:
1609
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene contains three cysteine-rich domains, a proline-rich region, and a pleckstrin homology domain with an overlapping Ras-associating domain. It is localized in the speckle domains of the nucleus, and mediates the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-175

List Size:
100 uL

List Price:

$419.00

Out of stock

Datasheet
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