Western blot analysis of extracts of various cell lines, using DHODH antibody (19-177).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

DHODH Antibody

CATALOG NUMBER: 19-177

Clonality:

Polyclonal

Tested Applications:

Host Species:

Rabbit

Species Reactivity:

Human, Mouse, Rat

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human, Mouse, Rat

Immunogen:

A synthetic peptide of human DHODH

Conjugate:

Unconjugated

Tested Applications:

Application Note:

WB: 1:500 - 1:5000

Positive Control 1:

MCF7

Positive Control 2:

Mouse spleen

Positive Control 3:

Rat spleen

Predicted Molecular Weight:

Observed: 43kDa

Advanced Validation

Properties

Purification:

Affinity purification

Clonality:

Polyclonal

Isotype:

IgG

Physical State:

Liquid

Buffer:

PBS with 0.02% sodium azide, pH7.3.

Concentration:

batch dependent

Storage Conditions:

Store at 4°C. Avoid freeze / thaw cycles.

Additional Info

Ncbi Official Symbol:

DHODH

Additional Names:

DHODH, DHOdehase, URA1, POADS

Ncbi Gene Id Number:

1723

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane.

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-177

List Size:

100 uL

List Price:

$365.00

Out of stock

Datasheet

Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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DHODH Antibody

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Positive Control 1:

Positive Control 2:

Positive Control 3:

Predicted Molecular Weight:

Purification:

Clonality:

Isotype:

Physical State:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Ncbi Gene Id Number:

User Note:

Background:

Disclaimer:

List Size:

Shipping Info

Customize your order

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