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Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.

DHODH Antibody

CATALOG NUMBER: 29-675

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DHODH.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
DHODH antibody can be used for detection of DHODH by ELISA at 1:62500. DHODH antibody can be used for detection of DHODH by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
43 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store DHODH antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
DHODH
Additional Names:
DHODH, DHOdehase, URA1, POADS
Protein Accession Number:
NP_001352
Protein Gi Number:
45006951
Ncbi Gene Id Number:
1723
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DHODH catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane.The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Background Reference 1:
Kimura, K., (2006) Genome Res. 16 (1), 55-65.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-675

List Size:
100 ul

List Price:

$519.00

Datasheet
Shipping Info

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