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Western Blot at 1:1000 dilution Lane 1: A375 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in HepG2 cell line and mouse liver tissue lysates (35ug/lane).
Western blot analysis of DHRS3 using rabbit polyclonal DHRS3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DHRS3 gene.
Western Blot at 1:1000 dilution Lane 1: A375 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

DHRS3 Antibody

CATALOG NUMBER: 63-828

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This DHRS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85-113 amino acids from the Central region of human DHRS3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
34 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DHRS3
Additional Names:
Short-chain dehydrogenase/reductase 3, DD831, Retinal short-chain dehydrogenase/reductase 1, retSDR1, DHRS3
Protein Accession Number:
O75911
Ncbi Gene Id Number:
9249
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DHRS3 catalyze the oxidation/reduction of a wide range of substrates, including retinoids and steroids.
Background Reference 1:
Persson,B., et.al., Chem. Biol. Interact. 178 (1-3), 94-98 (2009)
Background Reference 2:
Roni,V., et.al., BMC Genomics 8, 42 (2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-828

List Size:
400 ul

List Price:

$495.00

Datasheet
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