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Western blot analysis of extracts of various cell lines, using DHRS9 antibody (22-128) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit._Exposure time: 390s.
Western blot analysis of extracts of various cell lines, using DHRS9 antibody (22-128) at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using DHRS9 Antibody (22-128) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using DHRS9 Antibody (22-128) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using DHRS9 antibody (22-128). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using DHRS9 antibody (22-128) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit._Exposure time: 390s.

DHRS9 Antibody

CATALOG NUMBER: 22-128

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 18-319 of human DHRS9 (NP_001135743.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:10 - 1:100
Positive Control 1:
THP-1
Positive Control 2:
Mouse trachea
Positive Control 3:
HT-29
Positive Control 4:
Mouse pancreas
Positive Control 5:
Rat trachea
Predicted Molecular Weight:
Observed: 35kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
DHRS9
Additional Names:
3-alpha-HSD, 3ALPHA-HSD, RDH-E2, RDH-TBE, RDH15, RDHL, RDHTBE, RETSDR8, SDR9C4, dehydrogenase/reductase SDR family member 9, 3-alpha hydroxysteroid dehydrogenase, NADP-dependent retinol dehydrogenase/reductase, dehydrogenase/reductase (SDR family) member 9, retinol dehydrogenase 15, retinol dehydrogenase homolog, short chain dehydrogenase/reductase family 9C member 4, short-chain dehydrogenase/reductase retSDR8, tracheobronchial epithelial cell-specific retinol dehydrogenase
Ncbi Gene Id Number:
10170
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the short-chain dehydrogenases/reductases (SDR) family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. This protein demonstrates oxidoreductase activity toward hydroxysteroids and is able to convert 3-alpha-tetrahydroprogesterone to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone in the cytoplasm, and may additionally function as a transcriptional repressor in the nucleus. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-128

List Size:
100 uL

List Price:

$436.00

Datasheet
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