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DHX36 Antibody

CATALOG NUMBER: 7163

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Rabbit polyclonal DHX36 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DHX36.
The immunogen is located within the last 50 amino acids of DHX36.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
DHX36 antibody can be used for detection of DHX36 by Western blot at 0.5 - 1 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Specificity:
At least four isoforms of DDX36 are known to exist; this antibody will detect the three longest isoforms.
Positive Control 1:
Cat. No. 1404 - Mouse Liver Tissue Lysate
Positive Control 2:
Cat. No. 10-201 - Human Liver Tissue Slide
Predicted Molecular Weight:
Predicted: 111 kDa
Purification:
DHX36 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
DHX36 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
DHX36 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
DHX36
Additional Names:
DHX36 Antibody: G4R1, RHAU, DDX36, MLEL1, KIAA1488, Probable ATP-dependent RNA helicase DHX36, DEAH box protein 36
Protein Accession Number:
NP_065916
Protein Gi Number:
167830433
Ncbi Gene Id Number:
170506
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DHX36 Antibody: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure. DHX36 (DEAH box protein 36), also known as MLE-like protein 1 and RNA helicase associated with AU-rich element ARE (RHAU), belongs to RNA helicase of the DEAH family and may function in sex development and spermatogenesis. It is expressed in testis and is evolutionary conserved with true orthologs in almost all animal species. DHX36 plays a role in degradation and deadenylation of mRNAs containing in their 3'-UTR the consensus ARE sequence element. DHX36 is required for early embryogenesis.
Background Reference 1:
Cordin O, Banroques J, Tanner NK, et al. The DEAD-box protein family of RNA helicases. Gene 2006; 367:17-37.
Background Reference 2:
Linder P. Dead-box proteins: a family affair—active and passive players in RNP-remodeling. Nucleic Acids Res. 2006; 34:4168-80.
Background Reference 3:
Lattmann S, Giri B, Vaughn JP, et al. Role of the amino terminal RHAU-specific motif in the recognition and resolution of guanine quadruplex-RNA by the DEAH-box RNA helicase RHAU. Nucleic Acids Res. 2010; 38:6219-33.
Background Reference 4:
Fu JJ, Li LY, Liu SF, et al. Expression research for human DDX36 and mouse Ddx36 gene in the adult testis. Yi Chuan Xue Bao 2003; 30: 201-208.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7163

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

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